Saturday, June 24, 2017

5.6: Describe a Simple Experiment to Investigate Carbon Dioxide Production by Yeast, in Different Conditions

EQUATION FOR ANAEROBIC RESPIRATION (YEAST):

Glucose       →       Ethanol      +      Carbon Dioxide


EXPERIMENT:

INVESTIGATING CARBON DIOXIDE PRODUCTION BY YEAST
Diagram showing Carbon Dioxide Production by Yeast
METHOD:

  • Mix Yeast with Sugar Solution in a Boiling Tube with a Layer of Oil to Prevent Oxygen from Entering (Prevents Aerobic Respiration)
  • Connect this Boiling Tube with A Test Tube of Limewater using a Capillary Tube and Enclose with a Bung
  • Place Boiling Tube with Yeast and Sugar Solution into Water Bath at Set Temperature and Count the Number of Bubbles Produced in Fixed Time
  • Change Temperature of Water Bath and Repeat
  • Compare Results at Different Temperatures

RESULTS:

  • Higher the Temperature, the More Bubbles of Carbon Dioxide will be Produced as Higher Temperatures will be Closer to Optimum Temperature of Enzymes in Yeast, Increasing Enzyme Activity
  • As Enzyme Activity Increases, Rate of Anaerobic Respiration will Increase
  • But if Temperature is Too High, Enzymes will Denature causing Carbon Dioxide Production to Stop

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